Antibodies and lectins: Difference between revisions
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=External Antibody Resources= | |||
[[Antibody | <em>'''CiteAb'''</em> | ||
[http://www.citeab.co.uk/ CiteAb] is an antibody search engine which allows users to search over 500,000 antibodies from 50 companies including Xenopus-specific antibodies. | |||
For antibodies used in Xenopus laevis papers click [http://www.citeab.co.uk/search?r=1 here]. | |||
For antibodies used in Xenopus tropicalis papers click [http://www.citeab.co.uk/search?r=44 here]. | |||
'''Biocompare''' | |||
[http://www.biocompare.com/Antibodies/ Biocompare] provides another search of commercial antibodies. | |||
=Xenbase Antibody Feature= | |||
Antibodies are now integrated with the entire database and can be searched here: [http://www.xenbase.org/reagents/antibody.do Antibody Search] | |||
A link to our legacy spreadsheet is here: [https://docs.google.com/spreadsheet/ccc?key=0At9P6Lmc0aQ1dDlTWU9MUlpjYURXQ2dMd2taMk92MlE Antibody spreadsheet] | |||
'''Unverified Candidates For Xenopus-Reactive Antibodies''' | |||
Antibodies listed in this [https://docs.google.com/spreadsheet/ccc?key=0Aj7_wi3dDdEfdEJ5WHpVNDlMaXBuZTBMUWtaZnZnbWc#gid=3 spreadsheet] are either listed for use with Xenopus by the manufacturer OR have significant antigen sequence similarity to Xenopus species. Note that these antibodies '''have NOT been tested by a Xenopus researcher to our knowledge.''' However, many of the manufacturers offer incentives for testing their antibodies in exchange for your positive/negative testing results. | |||
=Common Xenopus Antibodies= | |||
'''Tissue-specific Antibodies''' | |||
[[12/101 - somites, skeletal muscle marker]] | [[12/101 - somites, skeletal muscle marker]] | ||
| Line 8: | Line 33: | ||
[[4A6 – pronephros]] | [[4A6 – pronephros]] | ||
[[5A3 - somite and heart]] | |||
[[zn-12 - neuronal marker]] | |||
'''A-D''' | |||
[[C-cadherin - cdh3]] | |||
[[E-cadherin - cdh1]] | |||
[[caspase 3, cleaved (asp175) - casp3]] | |||
[[alpha-catenin - ctnna1]] | |||
[[beta-catenin, active - ctnnb1]] | |||
[[beta-catenin - ctnnb1 - H-102]] | |||
[[beta-catenin, phosphorylated - ctnnb1]] | |||
[[CBFβ - cbfb - blood, CNS, blood vessels]] | |||
[[centrin 1 - cetn1 - basal bodies]] | [[centrin 1 - cetn1 - basal bodies]] | ||
| Line 13: | Line 61: | ||
[[collagen type II - col2a1 - ECM]] | [[collagen type II - col2a1 - ECM]] | ||
[[collagen type II - col2a1 - ECM]] | [[collagen type II - col2a1 - ECM broad]] | ||
'''E-L''' | |||
[[eya1 - eya1 - developing ear]] | |||
[[fibrillin-2 - fbn2 - secreted from endothelium]] | [[fibrillin-2 - fbn2 - secreted from endothelium]] | ||
[[ | [[anti-FLAG M2 monoclonal antibody]] | ||
[[flk1 - VEGFR2 - kdr - endothelium, blood]] | |||
[[anti-green fluorescent protein (GFP)]] | |||
[[histone H3, phosphorylated - h3f3a - from cell signaling techonology]] | |||
[[histone H3, phosphorylated - h3f3a - mitotic cells - from upstate]] | |||
[[hnk-1 - b3gat1 - migrating neural crest]] | |||
[[integrin, beta1 - itgb1]] | |||
[[JNK, activated - mapk8]] | |||
[[laminin - lama1]] | |||
[[LMO2 - lmo2 - blood, presomitic mesoderm, CNS]] | |||
'''M-S''' | |||
[[p44/42 MAPK, ERK1/2 phosphorylated - mapk1 - from cell signaling technology]] | |||
[[p44/42 MAPK, ERK1/2 phosphorylated - mapk1 - from sigma]] | |||
[[p44/42 MAPK, ERK1/2 phosphorylated - mapk1 - from sigma (2)]] | |||
[[p44/42 MAPK, ERK1/2 phosphorylated, alkaline phosphatase conjugate - mapk1 - from sigma]] | |||
[[c-myc - myc - from roche]] | |||
[[c-myc - myc - from sigma]] | |||
[[anti-c-myc-peroxidase conjugate - myc]] | |||
[[PKC mu - prkd1]] | |||
[[aPKC - PKC zeta - prkcz]] | |||
[[slug - snai2 - endothelial to mesenchyme transition, neural crest]] | |||
[[smad1 - smad1 - from abcam]] | |||
[[smad1 - smad1 - from invitrogen]] | |||
[[smad 1/5/8, phosphorylated - smad1 - smad8]] | |||
'''T-Z''' | |||
[[tcf-4 - tcf4 - from upstate]] | |||
[[troponin - heart and other muscle]] | [[troponin - heart and other muscle]] | ||
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[[alpha-tubulin - tuba4b - microtubules]] | [[alpha-tubulin - tuba4b - microtubules]] | ||
[[alpha-tubulin, acetylated - tuba4b]] | |||
[[gamma-tubulin - tubg1 - basal bodies, microtubule minus ends]] | [[gamma-tubulin - tubg1 - basal bodies, microtubule minus ends]] | ||
[[VEGFA - vegfa - somites, lateral plate mesoderm, head, hypochord]] | |||
[[ZO-1 - tjp1]] | [[ZO-1 - tjp1]] | ||
| Line 40: | Line 146: | ||
[[PNA - cement gland and scattered epidermal cells]] | [[PNA - cement gland and scattered epidermal cells]] | ||
[[Antibody Reagent Template]] | |||
Latest revision as of 13:46, 26 June 2017
External Antibody Resources
CiteAb
CiteAb is an antibody search engine which allows users to search over 500,000 antibodies from 50 companies including Xenopus-specific antibodies.
For antibodies used in Xenopus laevis papers click here. For antibodies used in Xenopus tropicalis papers click here.
Biocompare
Biocompare provides another search of commercial antibodies.
Xenbase Antibody Feature
Antibodies are now integrated with the entire database and can be searched here: Antibody Search
A link to our legacy spreadsheet is here: Antibody spreadsheet
Unverified Candidates For Xenopus-Reactive Antibodies
Antibodies listed in this spreadsheet are either listed for use with Xenopus by the manufacturer OR have significant antigen sequence similarity to Xenopus species. Note that these antibodies have NOT been tested by a Xenopus researcher to our knowledge. However, many of the manufacturers offer incentives for testing their antibodies in exchange for your positive/negative testing results.
Common Xenopus Antibodies
Tissue-specific Antibodies
12/101 - somites, skeletal muscle marker
3G8- pronephros and otic vesicle
A-D
caspase 3, cleaved (asp175) - casp3
beta-catenin, phosphorylated - ctnnb1
CBFβ - cbfb - blood, CNS, blood vessels
centrin 1 - cetn1 - basal bodies
collagen type II - col2a1 - ECM
collagen type II - col2a1 - ECM broad
E-L
fibrillin-2 - fbn2 - secreted from endothelium
anti-FLAG M2 monoclonal antibody
flk1 - VEGFR2 - kdr - endothelium, blood
anti-green fluorescent protein (GFP)
histone H3, phosphorylated - h3f3a - from cell signaling techonology
histone H3, phosphorylated - h3f3a - mitotic cells - from upstate
hnk-1 - b3gat1 - migrating neural crest
LMO2 - lmo2 - blood, presomitic mesoderm, CNS
M-S
p44/42 MAPK, ERK1/2 phosphorylated - mapk1 - from cell signaling technology
p44/42 MAPK, ERK1/2 phosphorylated - mapk1 - from sigma
p44/42 MAPK, ERK1/2 phosphorylated - mapk1 - from sigma (2)
p44/42 MAPK, ERK1/2 phosphorylated, alkaline phosphatase conjugate - mapk1 - from sigma
anti-c-myc-peroxidase conjugate - myc
slug - snai2 - endothelial to mesenchyme transition, neural crest
smad1 - smad1 - from invitrogen
smad 1/5/8, phosphorylated - smad1 - smad8
T-Z
troponin - heart and other muscle
troponin - cardiac troponin I - tnni3 - heart
troponin - cardiac troponin T - tnnt2 - heart
alpha-tubulin - tuba4b - microtubules
alpha-tubulin, acetylated - tuba4b
gamma-tubulin - tubg1 - basal bodies, microtubule minus ends
VEGFA - vegfa - somites, lateral plate mesoderm, head, hypochord
Lectins
LEL - all pronephric epithelia- also faint lateral line
PNA - cement gland and scattered epidermal cells