Antibodies and lectins: Difference between revisions
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*note that the antibody spreadsheet is currently being updated for the upcoming Xenbase upload and therefore, the entries are not in a user-friendly order. | *note that the antibody spreadsheet is currently being updated for the upcoming Xenbase upload and therefore, the entries are not in a user-friendly order. | ||
'''Biocompare''' | |||
[http://www.biocompare.com/Antibodies/ Biocompare] provides another search of commercial antibodies. | |||
'''Unverified candidates for Xenopus-reactive antibodies''' | |||
Antibodies listed in this [https://docs.google.com/spreadsheet/ccc?key=0Aj7_wi3dDdEfdEJ5WHpVNDlMaXBuZTBMUWtaZnZnbWc#gid=3 spreadsheet] are either listed for use with Xenopus by the manufacturer OR have significant antigen sequence similarity to Xenopus species. Note that these antibodies '''have NOT been tested by a Xenopus researcher to our knowledge.''' However, many of the manufacturers offer incentives for testing their antibodies in exchange for your positive/negative testing results. | |||
=Common Xenopus Antibodies= | =Common Xenopus Antibodies= | ||
Revision as of 08:11, 25 January 2013
External Antibody resources
CiteAb
[CiteAb is an antibody search engine which allows users to search over 500,000 antibodies from 50 companies including Xenopus-specific antibodies.
For antibodies used in Xenopus laevis papers click here. For antibodies used in Xenopus tropicalis papers click here.
Upcoming Xenbase Antibody Feature
A number of antibodies have been documented as useful for Xenopus studies but are not yet uploaded to Xenbase...
...see the complete list in this spreadsheet
- note that the antibody spreadsheet is currently being updated for the upcoming Xenbase upload and therefore, the entries are not in a user-friendly order.
Biocompare
Biocompare provides another search of commercial antibodies.
Unverified candidates for Xenopus-reactive antibodies
Antibodies listed in this spreadsheet are either listed for use with Xenopus by the manufacturer OR have significant antigen sequence similarity to Xenopus species. Note that these antibodies have NOT been tested by a Xenopus researcher to our knowledge. However, many of the manufacturers offer incentives for testing their antibodies in exchange for your positive/negative testing results.
Common Xenopus Antibodies
Tissue-specific Antibodies
12/101 - somites, skeletal muscle marker
3G8- pronephros and otic vesicle
A-D
caspase 3, cleaved (asp175) - casp3
beta-catenin, phosphorylated - ctnnb1
CBFβ - cbfb - blood, CNS, blood vessels
centrin 1 - cetn1 - basal bodies
collagen type II - col2a1 - ECM
collagen type II - col2a1 - ECM broad
E-L
fibrillin-2 - fbn2 - secreted from endothelium
anti-FLAG M2 monoclonal antibody
flk1 - VEGFR2 - kdr - endothelium, blood
anti-green fluorescent protein (GFP)
histone H3, phosphorylated - h3f3a - from cell signaling techonology
histone H3, phosphorylated - h3f3a - mitotic cells - from upstate
hnk-1 - b3gat1 - migrating neural crest
LMO2 - lmo2 - blood, presomitic mesoderm, CNS
M-S
p44/42 MAPK, ERK1/2 phosphorylated - mapk1 - from cell signaling technology
p44/42 MAPK, ERK1/2 phosphorylated - mapk1 - from sigma
p44/42 MAPK, ERK1/2 phosphorylated - mapk1 - from sigma (2)
p44/42 MAPK, ERK1/2 phosphorylated, alkaline phosphatase conjugate - mapk1 - from sigma
anti-c-myc-peroxidase conjugate - myc
slug - snai2 - endothelial to mesenchyme transition, neural crest
smad1 - smad1 - from invitrogen
smad 1/5/8, phosphorylated - smad1 - smad8
T-Z
troponin - heart and other muscle
troponin - cardiac troponin I - tnni3 - heart
troponin - cardiac troponin T - tnnt2 - heart
alpha-tubulin - tuba4b - microtubules
alpha-tubulin, acetylated - tuba4b
gamma-tubulin - tubg1 - basal bodies, microtubule minus ends
VEGFA - vegfa - somites, lateral plate mesoderm, head, hypochord
Lectins
LEL - all pronephric epithelia- also faint lateral line
PNA - cement gland and scattered epidermal cells