Antibodies and lectins: Difference between revisions
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=External Antibody resources= | |||
A number of antibodies have been documented but are not yet uploaded to Xenbase... | '''CiteAb''' | ||
[[http://www.citeab.co.uk/ CiteAb] is an antibody search engine which allows users to search over 500,000 antibodies from 50 companies including Xenopus-specific antibodies. | |||
For antibodies used in Xenopus laevis papers click [http://www.citeab.co.uk/search?r=1 here]. | |||
For antibodies used in Xenopus tropicalis papers click [http://www.citeab.co.uk/search?r=44 here]. | |||
'''Upcoming Xenbase Antibody Feature''' | |||
A number of antibodies have been documented as useful for Xenopus studies but are not yet uploaded to Xenbase... | |||
...see the complete list in this [https://docs.google.com/spreadsheet/ccc?key=0At9P6Lmc0aQ1dDlTWU9MUlpjYURXQ2dMd2taMk92MlE spreadsheet] | ...see the complete list in this [https://docs.google.com/spreadsheet/ccc?key=0At9P6Lmc0aQ1dDlTWU9MUlpjYURXQ2dMd2taMk92MlE spreadsheet] | ||
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=Common Xenopus Antibodies= | |||
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[[Antibody Reagent Template]] | |||
Revision as of 07:58, 25 January 2013
External Antibody resources
CiteAb
[CiteAb is an antibody search engine which allows users to search over 500,000 antibodies from 50 companies including Xenopus-specific antibodies.
For antibodies used in Xenopus laevis papers click here. For antibodies used in Xenopus tropicalis papers click here.
Upcoming Xenbase Antibody Feature
A number of antibodies have been documented as useful for Xenopus studies but are not yet uploaded to Xenbase...
...see the complete list in this spreadsheet
- note that the antibody spreadsheet is currently being updated for the upcoming Xenbase upload and therefore, the entries are not in a user-friendly order.
Common Xenopus Antibodies
Tissue-specific Antibodies
12/101 - somites, skeletal muscle marker
3G8- pronephros and otic vesicle
A-D
caspase 3, cleaved (asp175) - casp3
beta-catenin, phosphorylated - ctnnb1
CBFβ - cbfb - blood, CNS, blood vessels
centrin 1 - cetn1 - basal bodies
collagen type II - col2a1 - ECM
collagen type II - col2a1 - ECM broad
E-L
fibrillin-2 - fbn2 - secreted from endothelium
anti-FLAG M2 monoclonal antibody
flk1 - VEGFR2 - kdr - endothelium, blood
anti-green fluorescent protein (GFP)
histone H3, phosphorylated - h3f3a - from cell signaling techonology
histone H3, phosphorylated - h3f3a - mitotic cells - from upstate
hnk-1 - b3gat1 - migrating neural crest
LMO2 - lmo2 - blood, presomitic mesoderm, CNS
M-S
p44/42 MAPK, ERK1/2 phosphorylated - mapk1 - from cell signaling technology
p44/42 MAPK, ERK1/2 phosphorylated - mapk1 - from sigma
p44/42 MAPK, ERK1/2 phosphorylated - mapk1 - from sigma (2)
p44/42 MAPK, ERK1/2 phosphorylated, alkaline phosphatase conjugate - mapk1 - from sigma
anti-c-myc-peroxidase conjugate - myc
slug - snai2 - endothelial to mesenchyme transition, neural crest
smad1 - smad1 - from invitrogen
smad 1/5/8, phosphorylated - smad1 - smad8
T-Z
troponin - heart and other muscle
troponin - cardiac troponin I - tnni3 - heart
troponin - cardiac troponin T - tnnt2 - heart
alpha-tubulin - tuba4b - microtubules
alpha-tubulin, acetylated - tuba4b
gamma-tubulin - tubg1 - basal bodies, microtubule minus ends
VEGFA - vegfa - somites, lateral plate mesoderm, head, hypochord
Lectins
LEL - all pronephric epithelia- also faint lateral line
PNA - cement gland and scattered epidermal cells